Access
To read this story in full you will need to login or make a payment (see right).
Letter
Nature 440, 556-560 (23 March 2006) | doi:10.1038/nature04518; Received 31 August 2005; Accepted 15 December 2005
Open Innovation Challenges
-
Protect Enzyme from In Planta Degradation
A proposal for stable expression of an enzyme in corn seed is desired.
-
Fast Growth of Transformed Soybean Shoots
A method for accelerating growth of soybean shoots is desired.
nature jobs
Two year postdoctoral position in ethics, health and law
- University Paris Descartes
- Paris, 75 006, France
Research Scientist for Analytical Development
- Novo Nordisk
- Bagsværd, Denmark
RNA-mediated response to heat shock in mammalian cells
Ilya Shamovsky1,2, Maxim Ivannikov1, Eugene S. Kandel3, David Gershon2,4 & Evgeny Nudler1
- Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
- Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
- The Cleveland Clinic Foundation Lerner Research Institute, Cleveland, Ohio 44195, USA
- Redox Pharmaceutical Corp, Greenvale, New York 11548, USA
Correspondence to: David Gershon2,4Evgeny Nudler1 Correspondence and requests for materials should be addressed to E.N. (Email: evgeny.nudler@med.nyu.edu) or D.G. (Email: dgershon@redoxpharm.com).
Abstract
The heat-shock transcription factor 1 (HSF1) has an important role in the heat-shock response in vertebrates by inducing the expression of heat-shock proteins (HSPs) and other cytoprotective proteins1. HSF1 is present in unstressed cells in an inactive monomeric form and becomes activated by heat and other stress stimuli. HSF1 activation involves trimerization and acquisition of a site-specific DNA-binding activity2, 3, which is negatively regulated by interaction with certain HSPs4, 5, 6. Here we show that HSF1 activation by heat shock is an active process that is mediated by a ribonucleoprotein complex containing translation elongation factor eEF1A and a previously unknown non-coding RNA that we term HSR1 (heat shock RNA-1). HSR1 is constitutively expressed in human and rodent cells and its homologues are functionally interchangeable. Both HSR1 and eEF1A are required for HSF1 activation in vitro; antisense oligonucleotides or short interfering (si)RNA against HSR1 impair the heat-shock response in vivo, rendering cells thermosensitive. The central role of HSR1 during heat shock implies that targeting this RNA could serve as a new therapeutic model for cancer, inflammation and other conditions associated with HSF1 deregulation.
To read this story in full you will need to login or make a payment (see right).
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
RESEARCH
DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicaseNature Structural Biology Correspondence (01 Sep 1996)
Verification of enhancement of the CSF space, not parenchyma, in acute stroke patients with early blood?brain barrier disruptionJournal of Cerebral Blood Flow & Metabolism Brief Communication
MRI of monocyte infiltration in an animal model of neuroinflammation using SPIO-labeled monocytes or free USPIOJournal of Cerebral Blood Flow & Metabolism Original Article
A GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteinsNature Immunology Article (01 Jun 2001)
See all 71 matches for Research
