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Nature 440, E2-E3 (9 March 2006) | doi:10.1038/nature04686; Published online 8 March 2006

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Molecular genetics: Verification that Snuppy is a clone

Jung Bin Lee2 & Chankyu Park3 for Seoul National University Investigation Committee

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Arising from: B. C. Lee et al. Nature 436, 641 (2005); see also communication from the H. G. Parker, L. Kruglyak and E. A. Ostrander

Somatic-cell nuclear-transfer technology has been used to clone a variety of animal species1, 2, 3, but the overall efficiency of the cloning process and the viability of embryos has remained low4. Until Lee et al. described the cloning of two Afghan hounds by nuclear transfer from adult skin fibroblasts into oocytes that had matured in vivo5, dog cloning had been unsuccessful because of the difficulty of collecting canine oocytes matured in vivo at metaphase II (ref. 6). Here we provide independent evidence from the Seoul National University Investigation Committee that Snuppy, the one of the pair to survive, is a genuine clone.

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