Supplementary information
From the following article:
Identification of pathways regulating cell size and cell-cycle progression by RNAi
Mikael Björklund, Minna Taipale, Markku Varjosalo, Juha Saharinen, Juhani Lahdenperä and Jussi Taipale
Nature 439, 1009-1013 (23 February 2006)
doi:10.1038/nature04469
Supplementary Methods
This file contains additional methods used in the study.
Supplementary Table 1
This file contains the identified components of pathways regulating cell cycle.
Supplementary Tables
This file contains Supplementary Tables 2–13.
Supplementary Figure Legends
This file contains text to accompany Supplementary Figures 1–12.
Supplementary Figure 1
An overview of the flow cytometry screen.
Supplementary Figure 2
Supplementary Figure 2 nature04469-s06.eps Micrographs from cells treated with dsRNAs targeting cell cycle-related genes.
Supplementary Figure 3
The flow cytometry data from kinase/phosphatase screen with standard errors from individual samples.
Supplementary Figure 4
The a) kinase and b) phosphatase dendrograms.
Supplementary Figure 5
Compares our data with the data of Bettencourt-Dias et al.
Supplementary Figure 6
The G1 and G2 phenotype of the samples in DGC screen.
Supplementary Figure 7
The correlation between cell size at G1 and G2 phases.
Supplementary Figure 8
This figure shows a) the apoptosis/cell death scores in DGC screen, b) representative micrographs from cells treated with dsRNA causing cell death, c). over-4N DNA content of treated cells from the DGC screen identifying cytokinesis and DNA replication defects, d) representative micrographs from cells treated with dsRNA causing over-4N DNA content.
Supplementary Figure S9
This figure shows the comparison of microscopy and MTS assay with flow cytometry.
Supplementary Figure 10
Analysis of yeast-two hybrid and genetic interactions in various RNAi screens.
Supplementary Figure 11
This file depicts western blots showing the effectiveness of RNAi.
Supplementary Figure S12
This file contains analysis of 'hit-rate' in selected protein complexes/pathways.
Supplementary Figure 13
Unsupervised hierarchical clustering of all the genes identified in DGC screen.
Supplementary Figure 14
This figure displays the expression pattern of the identified genes during Drosophila life cycle. Cyclin E, E2f and eIF-3p66 are indicated with an arrow.
Supplementary Figure 15
This figure shows the pathways regulating cell cycle in S2 cells.
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