FIGURE 4. Flag–JHDM1A-mediated histone demethylation generates formaldehyde and succinate.
From the following article:
Histone demethylation by a family of JmjC domain-containing proteins
Yu-ichi Tsukada, Jia Fang, Hediye Erdjument-Bromage, Maria E. Warren, Christoph H. Borchers, Paul Tempst and Yi Zhang
Nature 439, 811-816 (16 February 2006)
doi:10.1038/nature04433
Demethylation reactions using highly purified Flag–JHDM1A expressed in Sf9 cells and a K36me2 peptide (STGGV2mKKPHRY-C) were subjected to mass spectrometric analysis. a, b, ESI-MS detection of formaldehyde (a; [M + H]+theoretical = 31.0184) and succinate (b; [M + H]+theoretical = 119.0344) in the presence (upper panel) and absence (lower panel) of Flag–JHDM1A. c, ESI-MS/MS analysis of ions at m/z 119 from a standard solution of succinate (600 nM) (top panel), and a Flag–JHDM1A reaction sample (bottom panel). Suggested structures of the succinate fragment ions are shown on the MS/MS spectrum.