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Supplementary information

From the following article:

Stem cell engraftment at the endosteal niche is specified by the calcium-sensing receptor

Gregor B. Adams, Karissa T. Chabner, Ian R. Alley, Douglas P. Olson, Zbigniew M. Szczepiorkowski, Mark C. Poznansky, Claudine H. Kos, Martin R. Pollak, Edward M. Brown and David T. Scadden

Nature 439, 599-603 (2 February 2006)

doi:10.1038/nature04247

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Supplementary Figure 1

Quantitative PCR analysis of car expression. RNA was obtained from purified CD11b+, CD3+ and lin-c-Kit+Sca-1+ (KLS) or lin-c-Kit+ (KL) cells and cDNA was made. Expression of car was then determined by real time PCR. Expression levels of car are shown relative to the expression of hprt.

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Supplementary Figure 2

Cell cycle analysis of primitive bone marrow cells from mice transplanted with CaR+/+ or CaR-/- cells. Mononuclear cells from E17.5 fetal liver were transplanted into lethally irradiated wild-type hosts. Eight weeks following injection of the cells, the bone marrow was analyzed for cell cycle status.

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