FIGURE 3. MEK inhibition causes loss of D-cyclin expression, RB hypophosphorylation and G1 arrest in BRAF mutant cancer cells.
From the following article:
BRAF mutation predicts sensitivity to MEK inhibition
David B. Solit, Levi A. Garraway, Christine A. Pratilas, Ayana Sawai, Gad Getz, Andrea Basso, Qing Ye, Jose M. Lobo, Yuhong She, Iman Osman, Todd R. Golub, Judith Sebolt-Leopold, William R. Sellers & Neal Rosen
Nature 439, 358-362(19 January 2006)
doi:10.1038/nature04304
a, Immunoblot showing the kinetics of change in p-ERK, D-cyclin expression and RB in SKMEL28 cells treated with 1 
M CI-1040. b, CI-1040 induced a G1 growth arrest in BRAF mutant tumour cells but not in RAS/BRAF-WT breast cancer cells (BT-474 shown). c, d, CI-1040 induces apoptosis in some but not all cancer cell lines with the BRAF(V600E) mutation as measured by FACS analysis (c) and PARP cleavage (d).
