FIGURE 1. Gene expression patterns that predict oncogenic pathway deregulation.

a, Image intensity display of the expression levels of genes most highly weighted in the predictor differentiating GFP-expressing control cells from cells expressing the indicated oncogenic activity. Expression levels are standardized to zero mean and unit variance across samples, displayed with genes as rows and samples as columns, and colour coded to indicate high (red) or low (blue) expression levels. b, Scatter plots depicting the classification of samples based on the first three principal components (expression patterns) derived from each signature, as shown in a. The gene expression values for each signature were extracted from all experimental samples and mean centred, then single value decomposition analysis was applied across all samples. Colour coding for samples is as follows: Myc, blue; Ras, green; E2F3, purple; Src, yellow; -catenin, red. Samples representing the specific pathway being examined are circled.