Supplementary information
From the following article:
Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk
Peijian Zou, Nikos Pinotsis, Stephan Lange, Young-Hwa Song, Alexander Popov, Irene Mavridis, Olga M. Mayans, Mathias Gautel and Matthias Wilmanns
Nature 439, 229-233(12 January 2006)
doi:10.1038/nature04343
Supplementary Discussion
Summarization of previous data on structural assemblies of immunoglobulin (IG)-like domain containing proteins.
Supplementary Methods
Details on production of seleno-L-methionine (SeMet)–incorporated telethonin, cellular targeting of telethonin and its mutants, antibodies, confocal microscopy.
Supplementary Table 1
In vitro/in vivo titin-telethonin assembly and localisation data of four telethonin mutants are listed.
Supplementary Table 2
X-ray data collection and phasing statistics of the structure of the titin/telethonin complex are summarised.
Supplementary Figure 1
The structure of the (2:1) titin/telethonin complex is shown schematically.
Supplementary Figure 2
Confocal images of neonatal rat cardiomyocytes that were transiently transfected with six different variants of telethonin. Those variants in which the structure of one the two telethonin 
-hairpins was affected do not properly localise to the Z-disk.
Supplementary Figure 3
Titin/telethonin complex formation of several telethonin variants under in vitro conditions, as evidenced by native gel electrophoresis. Those variants in which the structure of one the two telethonin -hairpins was affected do allow complex formation.
Supplementary Figure 4
Titin/telethonin complex formation of several telethonin variants in COS cells using a YFP-complementation assay. Those variants in which the structure of one the two telethonin -hairpins was affected do not complement.
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