Supplementary information

From the following article:

Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts

Alexander Meissner and Rudolf Jaenisch

Nature 439, 212-215(12 January 2006)

doi:10.1038/nature04257

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Supplementary Figure 1

Selection of functional shRNAs. Knockdown efficiency of each shRNA (see Methods for design of shRNAs) was tested using a DsRed reporter construct.

Supplementary Figure 2

Knockdown of endogenous Cdx2. The knockdown efficiency of the shRNA selected by the reporter assay (Supplementary Fig. 1) against endogenous Cdx2 was tested using ZHBTc4 ES cells.

Supplementary Figure Legends

Text to accompany the above Supplementary Figures.

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