FIGURE 3. Differentiation of blastomere-derived mES cells in vitro and in vivo.
From the following article:
Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
Young Chung, Irina Klimanskaya, Sandy Becker, Joel Marh, Shi-Jiang Lu, Julie Johnson, Lorraine Meisner and Robert Lanza
Nature 439, 216-219(12 January 2006)
doi:10.1038/nature04277
a, c, e, Immunofluorescence analysis of molecular markers of mesoderm (muscle actin, a), primitive endoderm (
-feto protein, c) and ectoderm (
III tubulin, e). g, Representative chromosome spreads of two single-blastomere-derived mES cell lines. b, Teratomas section, stained with haematoxylin and eosin. bn, bone (mesoderm); nt, neural tissue (ectoderm); cre and insert, ciliated respiratory epithelium (endoderm). d, f, h, 11.5 d.p.c. chimaeric embryos (d, f) and chimaeric pups (h) produced from three mES cell lines; arrow in f shows the extraembryonic mesoderm-derived placental labyrinth, also chimaeric. Scale bars: a, c, 100 
m; e, 200 m; d, 10 mm; f, 2 mm.
