FIGURE 1. Stages of single blastomere growth in the presence or absence of mES cells.
From the following article:
Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
Young Chung, Irina Klimanskaya, Sandy Becker, Joel Marh, Shi-Jiang Lu, Julie Johnson, Lorraine Meisner and Robert Lanza
Nature 439, 216-219(12 January 2006)
doi:10.1038/nature04277
a, b, Clump of GFP-positive mES cells 48 h after aggregation with single blastomeres; arrow shows a protruding cluster of GFP-negative cells. c, d, Outgrowth of GFP-negative cells aggregated with GFP-positive mES cells, after being plated on MEFs; arrows point to GFP-negative cells. e, f, Passage 1 of the outgrowth; arrows show remaining GFP-positive mES cells. g, h, Single blastomere outgrowth on MEFs for 4 days without ES cells, stained with Troma-1 and DAPI. a, c, e, Green fluorescence; b, Hoffman modulation optics; d, f, phase contrast. Scale bar, 100 
m.
