FIGURE 1. siRNAs administered intravaginally are efficiently taken up by vaginal tissue and durably silence endogenous EGFP expression.
From the following article:
An siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infection
Deborah Palliser, Dipanjan Chowdhury, Qing-Yin Wang, Sandra J. Lee, Roderick T. Bronson, David M. Knipe and Judy Lieberman
Nature 439, 89-94 (5 January 2006)
doi:10.1038/nature04263
a, FITC-siRNA mixed with Oligofectamine is efficiently taken up throughout the mucosa and submucosa. Sections were obtained 24 h after administration. b, siRNA targeting EGFP, but not an inverted control sequence, silences EGFP expression throughout the mouse vagina and cervix in GFP transgenic mice three days after administration. Liver EGFP expression is unaffected. c, Silencing persists for at least nine days in the vagina of GFP transgenic mice treated with GFP siRNAs. Data are representative of at least two experiments. An siRNA targeting an irrelevant gene (Set) was administered to control mice.