Supplementary information
From the following article:
Lipid–protein interactions in double-layered two-dimensional AQP0 crystals
Tamir Gonen, Yifan Cheng, Piotr Sliz, Yoko Hiroaki, Yoshinori Fujiyoshi, Stephen C. Harrison & Thomas Walz
Nature 438, 633-638(1 December 2005)
doi:10.1038/nature04321
Supplementary Notes
Lipid-protein and lipid-lipid interactions in the AQP0 junction, together with legends for supplementary figures.
Supplementary Figure 1
Electron diffraction pattern of a double-layered AQP0 2D crystal tilted to 60°.
Supplementary Figure 2
Protein packing in 3D and 2D crystals of AQP0.
Supplementary Figure 3
Residues in loop A of AQP0 involved in junction formation.
Supplementary Figure 4
The constricted water pore in junctional AQP0.
Supplementary Figure 6
Stereo view of the nine lipids surrounding an AQP0 monomer in the 2D crystals.
Supplementary Figure 7
Lipids surrounding the AQP0 tetramer mediate the crystal contacts.
Supplementary Table 1
Crystallographic statistics of non-junctional AQP0 (X-ray).
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