Supplementary information

From the following article:

Structure of the E. coli protein-conducting channel bound to a translating ribosome

Kakoli Mitra, Christiane Schaffitzel, Tanvir Shaikh, Florence Tama, Simon Jenni, Charles L. Brooks, III, Nenad Ban & Joachim Frank

Nature 438, 318-324(17 November 2005)

doi:10.1038/nature04133

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Supplementary Figure 1

Generation of RNC-PCC complexes.

Supplementary Figure 2

Selfrotation analysis of the non-translocating PCC EM density using the program GLRF.

Supplementary Figure 3

Normal mode analysis of the plug-less SecY complex.

Supplementary Figure 4

Normal mode-based flexible fitting (NMFF) of the plug-less SecY complex.

Supplementary Figure 5

Effect of resolution on the cryo-EM density of the translocating PCC.

Supplementary Notes

This file contains the Supplementary Results, Supplementary Discussion, Supplementary Figure Legends, Supplementary Methods and additional references.

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