Supplementary information

From the following article:

Pathogenic fungus harbours endosymbiotic bacteria for toxin production

Laila P. Partida-Martinez & Christian Hertweck

Nature 437, 884-888(6 October 2005)

doi:10.1038/nature03997

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Supplementary Figure 1

MSn spectrum of rhizoxin (1,) produced by cultivated symbiont. Inset shows diagnostic fork — shape UV spectrum of the triene moiety of 1.

Supplementary Figure 2

Generation of symbiont-free Rhizopus strain and reinfection with cultivated symbiont. Agarose (2 %) gel electrophoresis of 16S rDNA PCR products amplified with universal primers. As template served fungal metagenomic DNA of symbiont-free R. microsporus ATCC 62417 (lane 1), and R. microsporus ATCC 62417 after reinfection (lane 2). Lanes 3 and 4 are positive and negative controls, respectively.

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