Supplementary information
From the following article:
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme
Luke J. Higgins, Feng Yan, Pinghua Liu, Hung-wen Liu and Catherine L. Drennan
Nature 437, 838-844 (1 October 2005)
doi: 10.1038/nature03924
Supplementary Methods
Site-directed mutagenesis, protein purification, assays, crystallization and data collection.
Supplementary Figure S1
Stereoviews of HppE active sites displayed with 2Fo-Fc maps contoured from 1.0-1.5
.
Supplementary Figure S2
Structural insights into catalysis (stereoviews).
Supplementary Figure S3
Stereoviews of S-HPP-Co(II)-HppE active sites displayed with 2Fo-Fc maps contoured from 1.0-1.5.
Supplementary Tables
Supplementary Table S1 details data and refinement statistics for Co and Apo HppE structures. Supplementary Table S2 details data and refinement statistics for Fe HppE structures
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