Supplementary information
From the following article:
Prion protein remodelling confers an immediate phenotypic switch
Prasanna Satpute-Krishnan & Tricia R. Serio
Nature 437, 262-265(8 September 2005)
doi:10.1038/nature03981
Supplementary Video S1
Movie of Sup35-GFP in a [psi-] X [PSI+] zygote isolated in Fig. 2a.
Supplementary Video S2
Movie of Sup35-GFP in a [psi-] X [psi-] zygote isolated in Fig. 2b.
Supplementary Video S3
Movie of Sup35-GFP in a [psi-] X [PSI+] zygote isolated in Fig. 2c.
Supplementary Figure Legends
Legends to accompany the below Supplementary Figures.
Supplementary Figure S1
Unprocessed Gst-Sup35 fusions cannot propagate the prion state.
Supplementary Figure S2
Sup35[PSI+] and Sup35[psi-] are equally stable and long-lived.
Supplementary Figure S3
Protein profiles in transient tag strains.
Supplementary Figure S4
PMFA1 is rapidly repressed in diploids upon mating.
Supplementary Figure S5
Sup35-GFP functionally replaces Sup35 and acts independently as a prion.
Supplementary Figure S6
Confirmation of [PSI] state of zygotes.
Supplementary Figure S8
Fluorescence pattern confirms mating in phenotypic switch experiments.
Supplementary Figure S9
Single cell reporter for stop codon read-through.
Supplementary Figure S10
Confirmation of [PSI] state of zygotes.
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