FIGURE 4. c-Jun is required for intestinal cancer development in Apc^Min/+ mice.

a, Scheme of the floxed c-jun allele before and after cre recombination. Arrows indicate approximate position of PCR primers used. b, PCR analysis of genomic DNA. Br, brain; Ht, heart; In, intestine; Ki, Kidney; Lu, lung; Th, thymus; Tl, tail. c, Western blot analysis of Apc^Min/+; c-jun^fl/fl control and Apc^Min/+; c-jun^G intestines. d, e, Haematoxylin and eosin (H + E) staining of intestinal sections (18-week-old mice). The dashed circle in e indicates a cyst. f−k, Immunohistochemistry for c-Jun (f, g), -catenin (h, i) and CD44 (j, k) on intestinal sections. The dashed circle in g indicates a cyst; arrowheads in h and i indicate cells with nuclear -catenin staining. k, Schematic representation of a JNK-dependent interaction between c-Jun and TCF4/-catenin on the c-jun promoter.