FIGURE 3. Absence of c-Jun N-terminal phosphorylation attenuates intestinal cancer development in Apc^Min mice.

a, Kaplan−Meier survival plots of Apc^Min/+; c-jun^+/+ (n = 16), Apc^Min/+; JunAA/ + (n = 13) and Apc^Min/+; JunAA/JunAA (n = 10) mice. The x axis indicates the time (in weeks) until mice had to be killed due to severe weight loss and anaemia. b, c, Pictures of whole intestines. Dashed circles indicate adenomas. d, e, Haematoxylin and eosin (H + E) staining of intestinal sections. Dashed lines indicate intestinal adenomas. f, Average tumour number grouped according to intestinal location in Apc^Min/+; c-jun^+/+ (n = 100, white bars) and Apc^Min/+; JunAA/JunAA mice (n = 49, black bars) at the age of 11 weeks. g, Quantification (in per cent) of tumour size distribution. White columns, Apc^Min/+; c-jun^+/+ mice; black columns, Apc^Min/+; JunAA/JunAA mice. h, Average tumour size at the age of 11 weeks; P < 0.01. i, Quantification of the proliferation index. j, Northern blot analysis of c-jun and Gapdh expression (loading control). k, Western blot analysis of indicated proteins. Values in f, h, i are mean s.e.m.