FIGURE 2. The transfer of l-Val from CmaA to CmaD catalysed by CmaE.
From the following article:
Cryptic chlorination by a non-haem iron enzyme during cyclopropyl amino acid biosynthesis
Frédéric H. Vaillancourt, Ellen Yeh, David A. Vosburg, Sarah E. O'Connor and Christopher T. Walsh
Nature 436, 1191-1194 (25 August 2005)
doi:10.1038/nature03797
a, Time course of the reaction catalysed by CmaE with l-[14C]Val in the presence of CmaA and CmaD. Left, SDS–PAGE; right, autoradiogram. b, CmaE was incubated with l-[14C]Val in the presence of CmaA with or without CmaD (lanes 1 and 4). CmaE C105A was incubated with l-[14C]Val in presence of CmaA with or without CmaD (lanes 2 and 5). CmaE was incubated with l-[14C]Val in presence of CmaA S542A with or without CmaD (lanes 3 and 6). Left, SDS–PAGE; right, autoradiogram. All reactions were quenched after a 10-min incubation.