Supplementary information

From the following article:

Systematic analysis of genes required for synapse structure and function

Derek Sieburth, QueeLim Ch'ng, Michael Dybbs, Masoud Tavazoie, Scott Kennedy, Duo Wang, Denis Dupuy, Jean-François Rual, David E. Hill, Marc Vidal, Gary Ruvkun & Joshua M. Kaplan

Nature 436, 510-517(28 July 2005)

doi:10.1038/nature03809

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Supplementary Notes

References, including those for Methods and for Supplementary Table S2.

Supplementary Figure S1

Classification of the 2072 RNAi genes selected to screen for aldicarb resistance, and classification of the 185 positive genes from the RNAi screens

Supplementary Figure 2

Aldicarb and phorbol ester resistance of mutants corresponding to positive genes.

Supplementary Figure S3

Images of the subcellular localization of dorsal punctate proteins, their co localization with SNB-1 and their localization in unc-104 KIF1A mutants.

Supplementary Figure S4

Images of motoneuron expression patterns of synaptic proteins.

Supplementary Table S1

List of 2072 genes screened.

Supplementary Table S2

Complete list of positives from the aldicarb resistant (Ric) and dgk-1 suppressor (Dgk) screens.

Supplementary Table S3

Homologs of positive genes from the RNAi screens.

Supplementary Table S4

Validation of RNAi screens with known C. elegans neurotransmission mutants.

Supplementary Table S5

Aldicarb resistance of mutants corresponding to positive genes.

Supplementary Table S6

Summary of protein localization data.

Supplementary Table S7

Expression patterns of transcriptional fusions.

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