FIGURE 2. Gene-expression signature associated with lung metastasis.
From the following article:
Genes that mediate breast cancer metastasis to lung
Andy J. Minn, Gaorav P. Gupta, Peter M. Siegel, Paula D. Bos, Weiping Shu, Dilip D. Giri, Agnes Viale, Adam B. Olshen, William L. Gerald and Joan Massagué
Nature 436, 518-524 (28 July 2005)
doi: 10.1038/nature03799
a, Comparison of gene expression profiles of LM2 populations with parental cells identifies 113 probe sets that are correlated with lung metastatic activity. This signature clusters populations selected in vivo and SCPs into groups that resemble the LM2 cell lines (red bar along the bottom), the parental MDA-MB-231 cell line (green bar) or an intermediate group (blue bar). b, LM2 populations 4175 and 4142 were assayed for lung metastatic activity as measured by BLI and were compared with parental populations and various SCPs13. Plots show a quantification of the luminescence signal as a function of time. Results are means 
s.e.m. for each cohort. Colour-coding is as in a. c, Northern blot analysis of parental, LM0, LM1 and LM2 cell lines with a set of nine lung metastasis genes selected for functional validation, as well as four genes underexpressed in the lung-metastatic populations.
