Supplementary information
From the following article:
A substrate-specific inhibitor of protein translocation into the endoplasmic reticulum
Jennifer L. Garrison, Eric J. Kunkel, Ramanujan S. Hegde and Jack Taunton
Nature 436, 285-289 (14 July 2005)
doi: 10.1038/nature03821
Supplementary Figure S1
Effect of cotransin and nor-cotransin on protein expression in stimulated primary human cells.
Supplementary Figure S2
Effect of cotransin and nor-cotransin on cellular glycoprotein expression.
Supplementary Figure S3
Cotransin does not influence the translocation, glycosylation, membrane insertion, or topology of model membrane proteins synthesized in vitro.
Supplementary Figure S4
Cotransin's effects are independent of ER lumenal proteins.
Supplementary Figure S5
Analysis of VCAM1 and pPrl translocation in fractionated proteoliposomes.
Supplementary Figure S6
Purification of Sec61 complex / SR and preparation of minimal proteoliposome.
Supplementary Table 1
Additional full length proteins assayed for translocation in the presence of cotransin in vitro.
Supplementary Figure Legends
Text to accompany the above Supplementary Figures.
Supplementary Methods
Methods and References to accompany Supplementary Figures.
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