Supplementary information
From the following article:
Kinase-regulated quantal assemblies and kiss-and-run recycling of caveolae
Lucas Pelkmans and Marino Zerial
Nature 436, 128-133(7 July 2005)
doi:10.1038/nature03866
Supplementary Figure Legends
Legends to accompany Supplementary Figures S1-S4
Supplementary Figure S1
Determination of caveolar structure size and number of Cav1-GFP molecules.
Supplementary Figure S2
TIR-FM image of a HeLa cell stably expressing Cav1-mRFP and intensity-time plots of TIR-FM time-lapse sequences (200ms intervals) of caveolar vesicles.
Supplementary Figure S3
An experiment in which dual colour Epi-FM and TIR-FM is combined with pH quenching (recorded with 300ms intervals).
Supplementary Methods
Additional methods section of the manuscript.
Supplementary Video Legends
Supplementary Video S1
TIR-FM of a cav1-deficient fibroblast strongly over-expressing Cav1-GFP.
Supplementary Video S2
TIR-FM of a HeLa cell stably expressing Cav1-GFP. Frame intervals are 500 ms.
Supplementary Video S2a
Detailed recording of another HeLa cell expressing Cav1-GFP.
Supplementary Video S3
TIRF imaging of a 25x25 pixel region of interest of the plasma membrane in which docking of caveolar vesicles occurs. Time between images is 17ms.
Supplementary Video S3a
TIRF imaging of a 25x25 pixel region of interest of the plasma membrane in which docking of caveolar vesicles occurs. Time between images is 17ms.
Supplementary Video S3b
TIRF imaging of a 25x25 pixel region of interest of the plasma membrane in which docking of caveolar vesicles occurs. Time between images is 17ms.
Supplementary Video S4
Simultaneous dual colour TIR-FM of HeLa cells stably expressing Cav1-mRFP and having bound FITC-ChTxB (methods) at neutral pH.
Supplementary Video S4a
Video zoomed in on a docking caveolar vesicle directly after changing the extra-cellular pH to 4.0 (methods).
Supplementary Video S4b
Same circumstances as 4a, except that the FITC signal is on the right and the Cav1-mRFP signal on the left.
Supplementary Video S4c
Same circumstances as 4a, except that the FITC signal is on the right and the Cav1-mRFP signal on the left.
Supplementary Video S4d
Video zoomed in on a docking caveolar vesicle directly after changing the extra-cellular pH to 4.0 (methods).
Supplementary Video S5
Combined TIR-FM (green) and Epi-FM (red) sequence of a HeLa cell stably expressing Cav1-GFP.
Supplementary Video S5a
Zoomed in on a few caveolar vesicles displaying kiss-and-run interactions.
Supplementary Video S5b
The caveolar vesicle located in the right bottom will detach and rapidly move in a directional manner diagonally to the upper right corner where it transiently docks on the plasma membrane, before detaching again and interacting with another caveolar vesicle close-by, underneath the plasma membrane.
Supplementary Video S6
TIR-FM sequence of a Cav1-GFP expressing HeLa cell silenced for ARAF1.
Supplementary Video S7
TIR-FM sequence of a Cav1-GFP expressing HeLa cell silenced for SRC.
Supplementary Video S8
TIR-FM sequence of a Cav1-GFP expressing HeLa cell silenced for KIAA0999.
Supplementary Video S9
TIR-FM sequence of a Cav1-GFP expressing HeLa cell silenced for DYRK3.
Supplementary Video S10
TIR-FM sequence of a Cav1-GFP expressing HeLa cell treated with 1 
M Okadaic Acid. Note the extensive kiss-and-run dynamics of caveolar structures.
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