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Letter

Nature 436, 123-127 (7 July 2005) | doi:10.1038/nature03688; Received 22 February 2005; Accepted 25 April 2005

Rac1b and reactive oxygen species mediate MMP-3-induced EMT and genomic instability

Derek C. Radisky1, Dinah D. Levy1, Laurie E. Littlepage2, Hong Liu1, Celeste M. Nelson1, Jimmie E. Fata1, Devin Leake3, Elizabeth L. Godden3, Donna G. Albertson4, M. Angela Nieto5, Zena Werb2 & Mina J. Bissell1

  1. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
  2. Department of Anatomy, University of California, San Francisco, California 94143, USA
  3. Dharmacon Inc., Lafayette, Colorado 80026, USA
  4. Department of Laboratory Medicine, and Comprehensive Cancer Center, University of California, San Francisco, California 94143, USA
  5. Department of Developmental Neurobiology, Instituto de Neurociencias de Alicante, 03550 San Juan de Alicante, Spain

Correspondence to: Derek C. Radisky1Mina J. Bissell1 Correspondence and requests for materials should be addressed to M.J.B. (Email: mjbissell@lbl.gov) or D.C.R. (Email: dcradisky@lbl.gov).

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The tumour microenvironment can be a potent carcinogen, not only by facilitating cancer progression and activating dormant cancer cells, but also by stimulating tumour formation1. We have previously investigated stromelysin-1/matrix metalloproteinase-3 (MMP-3), a stromal enzyme upregulated in many breast tumours2, and found that MMP-3 can cause epithelial–mesenchymal transition (EMT) and malignant transformation in cultured cells3, 4, 5, and genomically unstable mammary carcinomas in transgenic mice3. Here we explain the molecular pathways by which MMP-3 exerts these effects: exposure of mouse mammary epithelial cells to MMP-3 induces the expression of an alternatively spliced form of Rac1, which causes an increase in cellular reactive oxygen species (ROS). The ROS stimulate the expression of the transcription factor Snail and EMT, and cause oxidative damage to DNA and genomic instability. These findings identify a previously undescribed pathway in which a component of the breast tumour microenvironment alters cellular structure in culture and tissue structure in vivo, leading to malignant transformation.

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