FIGURE 3. FISH, Kaplan–Meier and AQUA analysis of MITF in human melanoma samples.
From the following article:
Levi A. Garraway, Hans R. Widlund, Mark A. Rubin, Gad Getz, Aaron J. Berger, Sridhar Ramaswamy, Rameen Beroukhim, Danny A. Milner, Scott R. Granter, Jinyan Du, Charles Lee, Stephan N. Wagner, Cheng Li, Todd R. Golub, David L. Rimm, Matthew L. Meyerson, David E. Fisher and William R. Sellers
Nature 436, 117-122(7 July 2005)
doi:10.1038/nature03664
Green digoxin- and red SpectrumOrange (from Vysis)-labelled BAC probes detected the MITF locus and chromosome 3 centromere, respectively, in a melanoma tissue microarray. More than 50 nuclei were scored per amplified sample. a, b, A diploid case (a) and a case of MITF amplification (b) are shown. c, Kaplan–Meier survival analysis in metastatic melanoma patients with or without MITF amplification (log rank test, P = 0.024). d, e, AQUA images for MITF protein levels in non-amplified and highly amplified samples (
10 copies per cell). f, Mean and standard error of AQUA scores for MITF protein in non-amplified (left) and amplified (right) metastatic melanoma samples.
