Supplementary information
From the following article:
c-Myc-regulated microRNAs modulate E2F1 expression
Kathryn A. O'Donnell, Erik A. Wentzel, Karen I. Zeller, Chi V. Dang and Joshua T. Mendell
Nature 435, 839-843(9 June 2005)
doi:10.1038/nature03677
Supplementary Figure S1
Conservation of the miRNA binding sites in the 3' UTR of E2F1 mRNA and reporter assays demonstrating direct regulation of this transcript by miR-17-5p and miR-20a.
Supplementary Figure S2
Northern and western blot analysis of E2F1 and c-Myc mRNA and protein levels during a serum-stimulation time-course of primary human fibroblasts.
Supplementary Figure Legends
Legends to accompany the above Supplementary Figures.
Supplementary Table S1
Sequences of probes spotted on miRNA array.
Supplementary Table S2
Sequences of primers used in ChIP analysis of the miR-17 cluster.
Supplementary Methods
Additional information on methods used in this study, including the construction of E2F1 and PTEN luciferase reporter plasmids and the luciferase assays.
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