FIGURE 4. miR-17-5p and miR-20a regulate E2F1 translational yield.
From the following article:
c-Myc-regulated microRNAs modulate E2F1 expression
Kathryn A. O'Donnell, Erik A. Wentzel, Karen I. Zeller, Chi V. Dang and Joshua T. Mendell
Nature 435, 839-843(9 June 2005)
doi:10.1038/nature03677
a, Inhibition of miR-17-5p and miR-20a by 2'-O-methyl-oligoribonucleotides. Sensor or control luciferase constructs were transfected into HeLa cells alone (mock) or with the following oligonucleotides: scrambled nucleotide at 20 or 40 pmol, or 20 pmol of miR-17-5p or miR-20a antisense (AS), either individually (miR-17-5p AS or miR-20a AS) or pooled (miR-17-5p + 20a AS). The ratio of normalized sensor to control luciferase activity is shown. Error bars represent standard deviations. b, c, Western blot (b) and northern blot (c) analysis of E2F1 in antisense-treated HeLa cells. d, Northern blot analysis of miR-20 in transfected HeLa cells. e, f, Western blot (e) northern blot (f) analysis of E2F1 in transfected HeLa cells. g, h, Northern blot (g) and western blot (h) analysis of E2F1 in P493-6 cells. Fold changes shown are mean values derived from three experiments.