FIGURE 3. c-Myc binds directly to the mir-17 cluster genomic locus.
From the following article:
c-Myc-regulated microRNAs modulate E2F1 expression
Kathryn A. O'Donnell, Erik A. Wentzel, Karen I. Zeller, Chi V. Dang and Joshua T. Mendell
Nature 435, 839-843(9 June 2005)
doi:10.1038/nature03677
a, Schematic representation of the genomic interval encompassing the mir-17 cluster. Putative c-Myc binding sites are indicated (CACGTG or CATGTG); those in red are conserved between human and mouse. The location and structure of C13orf25 is indicated. Real-time PCR amplicons are represented by numbered lines. b, Real-time PCR analysis of c-Myc chromatin immunoprecipitates. Amplification of a validated c-Myc-binding site in intron 1 of the B23 gene served as a positive control22. c, Western blot analysis of c-Myc protein levels following serum stimulation of primary human fibroblasts. d, e, Real-time PCR analysis of mir-17 cluster expression (d) and c-Myc chromatin immunoprecipitates in serum-stimulated fibroblasts (e). Error bars for all panels represent standard deviations derived from at least three independent measurements.