FIGURE 2. c-Myc induces expression of the miR-17 cluster.
From the following article:
c-Myc-regulated microRNAs modulate E2F1 expression
Kathryn A. O'Donnell, Erik A. Wentzel, Karen I. Zeller, Chi V. Dang and Joshua T. Mendell
Nature 435, 839-843(9 June 2005)
doi:10.1038/nature03677
a, Schematic representation of the mir-17, mir-106a and mir-106b clusters. mir-18b and mir-20b are predicted on the basis of homology to mir-18a and mir-20a, respectively30. b, Northern blot analysis of miRNAs in P493-6 cells. Duplicate samples are shown, and miR-30 served as a loading control. Blots were also probed for miR-16 and miR-29 as loading controls, and similar results were obtained (data not shown). c, Northern blot analysis of total RNA from P493-6 cells with a probe specific for the mir-17 cluster. 7SK RNA served as a loading control. d, Northern blot analysis of miRNAs in wild-type rat fibroblasts (+ / + ), rat fibroblasts with a homozygous deletion of c-Myc (- / - ), or knockout fibroblasts reconstituted with wild-type c-Myc (- / - (Myc)). Quantification of radioactive signal intensity is shown on the right.