FIGURE 4. Nup358/RanBP2 activities.
From the following article:
Insights into E3 ligase activity revealed by a SUMO−RanGAP1−Ubc9−Nup358 complex
David Reverter and Christopher D. Lima
Nature 435, 687-692 (2 June 2005)
doi: 10.1038/nature03588
a, SUMO-1 RanGAP1 conjugation under multiple turnover with (open circles) or without (filled circles) IR1*; gel insets are shown. b, Inhibition of SUMO-1 p53 conjugation by SUMO-1−RanGAP1, with (filled circles) or without (open circles) IR1*; gel insets are shown. c, Multiple-turnover SUMO-1 conjugation rates for p53 tetramerization domain (left), I
B
(middle) and p53 peptide (right). d, Gel insets for c with p53. e, Rates (pM s-1) and relative rates for Nup358/RanBP2 constructs in c. f, Gel insets for g using p53. g, Single-turnover rates for p53 tetramerization domain (left) and IB (right) using Nup358/RanBP2 constructs or IR1-M−RanGAP1−Ubc9−SUMO−Nup358/RanBP2 (yellow). h, Rates (pM s-1) for g. i, Single-turnover rates for p53 without (red) or with (black) IR1*. Note the different scales in the three panels. Results in graphs are means 
s.d.