FIGURE 3. Nup358/RanBP2 sequence alignment and E2−E3−SUMO-1 structure.
From the following article:
Insights into E3 ligase activity revealed by a SUMO−RanGAP1−Ubc9−Nup358 complex
David Reverter and Christopher D. Lima
Nature 435, 687-692 (2 June 2005)
doi: 10.1038/nature03588
a, IR1-M-IR2 elements. Secondary structure is shown above the alignment. Single-letter amino-acid code is coloured for SUMO (yellow) and Ubc9 (blue) contacts. Nup358/RanBP2 IR constructs are indicated by bars. Mutational analysis18 is shown below IR1-M: double dagger, no defect; plus sign, impaired activity; minus sign, no activity. Asterisks above IR2 indicate identical amino-acid positions between IR1 and IR2. b, Nup358/RanBP2 motifs I−V (magenta), SUMO-1 (yellow) and Ubc9 (blue). c, Nup358/RanBP2 motif I. d, Nup358/RanBP2 motif II. e, Nup358/RanBP2 motif III. f, Nup358/RanBP2 motif IV. g, Nup358/RanBP2 motif V. Residues are labelled, and hydrogen-bonding interactions are indicated by dashed lines.