Supplementary information

From the following article:

Allosteric modulation of the presynaptic Ca2+ sensor for vesicle fusion

Xuelin Lou, Volker Scheuss & Ralf Schneggenburger

Nature 435, 497-501(26 May 2005)

doi:10.1038/nature03568

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Supplementary Figures

This file contains Supplementary Figures S1 and S2 and accompanying legends. Supplementary Figure S1 shows that EPSCs evoked by Ca2+-uncaging, and by brief presynaptic depolarizations are potentiated with a similar time-course by 1 microM PDBu, but the potentiation is transient under conditions of presynaptic whole-cell recording. Supplementary Figure S2 shows that EPSCs evoked by brief, and by prolonged presynaptic depolarizations are potentiated differentially by 1 microM PDBu, suggesting that the size of a readily-releasable vesicle pool is unchanged by PDBu. Furthermore, Ca2+-currents evoked by brief presynaptic depolarizations are slightly (approx15 %) increased by 1 microM PDBu.

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