Supplementary information

From the following article:

FGF-induced vesicular release of Sonic hedgehog and retinoic acid in leftward nodal flow is critical for left–right determination

Yosuke Tanaka, Yasushi Okada & Nobutaka Hirokawa

Nature 435, 172-177(12 May 2005)

doi:10.1038/nature03494

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Supplementary Movie S1

Leftward nodal flow of fluorescent beads irrespective of FGF signalling.

Supplementary Movie S2

Fluorescent images of mouse nodes in ventral views with unidirectional flow of NVPs towards the left.

Supplementary Movie S3

Ventral view of a mouse node, whose NVP flow is suppressed by 20 microM SU5402.

Supplementary Movie S4

Ball-throwing release of an NVP from the tip of a dynamically protruding microvillum of the right wall of a node.

Supplementary Movie S5

Crawling and smashed appearance of NVPs.

Supplementary Movie S6

Fragmentation of an NVP in the proximity of the left wall.

Supplementary Movie S7

Restored NVP flow by the addition of SHH-N peptide in the presence of SU5402.

Supplementary Movie S8

NVP flow is restored by the addition of 10-7M RA in the presence of SU5402.

Supplementary Movie S9

Ventral view of a node treated with SU5402 and IHH-N peptide.

Supplementary Movie S10

Ventral view of an iv/iv mutant node.

Supplementary Movie S11

Ventral view of a kif3a-/- mutant node lacking the nodal cilia.

Supplementary Movie 12

Rotating view of a mouse ventral node fluorescently labelled by 5E1 antibody against SHH-N.

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