Supplementary information
From the following article:
DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis
Jirina Bartkova,
Zuzana Ho
ej
í,
Karen Koed,
Alwin Krämer,
Frederic Tort,
Karsten Zieger,
Per Guldberg,
Maxwell Sehested,
Jahn M. Nesland,
Claudia Lukas,
Torben Ørntoft,
Jiri Lukas
and
Jiri Bartek
Nature 434, 864-870(14 April 2005)
doi:10.1038/nature03482
Supplementary Figure Legends
Legends to accompany the below Supplementary Figures.
Supplementary Figure S1
Immunostaining of 
H2AX in human Ta lesions of the urinary bladder, shown at high magnification by confocal laser microscope or immunoperoxidase staining to document the focal pattern of H2AX in tumour cell nuclei.
Supplementary Figure S2
Summary data for Ser1981-phosphorylated ATM, Thr68-phosphorylated Chk2, and classification of bladder tumours according to their genomic instability deduced from the SNP array analysis of allelic imbalances.
Supplementary Figure S3
Examples of immunohistochemistry images to illustrate that mononuclear cell infiltrate is insufficient to activate the DNA damage response in human colon tissues.
Supplementary Figure S4
Cytological detection (confocal laser microscope and light microscope images) of activated DNA damage checkpoint markers in U-2-OS cells exposed to various hyperproliferative, oncogenic stimuli.
Supplementary Figure S5
Examples of immunoperoxidase and immunofluorescence images of cyclin E-activated DNA damage checkpoint markers in normal human fibroblasts.
Supplementary Figure S6
Evidence for altered dynamics of DNA replication and chromatin association of the RPA protein) on induced overexpression of cyclin E in human U-2-OS cells.
Supplementary Figure S7
Immunohsitochemical evidence for aberrantly enhanced cyclin E and H2AX in colon adenomas and summary to document the correlation of -H2AX and elevated cyclin E.
Supplementary Figure S8
Examples of immunohistochemical patterns of Tyr15-phosphorylated Cdk1, Ki67 and H2AX in colon adenomas, and summary of frequencies and correlation between the H2AX and pTyr-Cdk1 markers in adenomas and carcinomas, respectively.
Supplementary Data
Data from the SNP array analysis of DNA isolated from the blood and microdissected lesions from samples of different stages of human bladder tumourigenesis, MIAME compliant.
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