FIGURE 5. THC reduces migration capacity and CCR2 expression in vitro.
From the following article:
Low dose oral cannabinoid therapy reduces progression of atherosclerosis in mice
Sabine Steffens, Niels R. Veillard, Claire Arnaud, Graziano Pelli, Fabienne Burger, Christian Staub, Andreas Zimmer, Jean-Louis Frossard and François Mach
Nature 434, 782-786(7 April 2005)
doi:10.1038/nature03389
a–d, Thioglycollate-elicited peritoneal cavity macrophages obtained from ApoE-/- (a, b) or CB2-/- (c, d) mice (n = 4 for both groups) were analysed for their in vitro migration response to the chemoattractant MCP-1. Cells were stimulated in duplicate with 1 ng ml-1 IFN-
(a, c) or 10 ng ml-1 TNF-
(b, d) in the presence or absence of 0.6 ng ml-1 THC and 1 
M of the CB2 antagonist SR144528. Data represent mean values 
s.e.m. Single asterisk, P < 0.05 (compared with IFN- or TNF--stimulated cells). e, Isolated splenocytes obtained from ApoE-/- mice (n = 4) were stimulated with 10 ng ml-1 TNF- in the presence or absence of different doses of THC (0.05–500 ng ml-1). Relative expression levels of CCR2 messenger RNA were determined by quantitative real-time PCR. Data represent mean values s.e.m. Single asterisk, P < 0.05 (compared with control, unstimulated cells); double asterisk, P < 0.05 (compared with TNF--stimulated cells).
