Supplementary information
From the following article:
Induction of photosensitivity by heterologous expression of melanopsin
Xudong Qiu, Tida Kumbalasiri, Stephanie M. Carlson, Kwoon Y. Wong, Vanitha Krishna, Ignacio Provencio and David M. Berson
Nature 433, 745-749(17 February 2005)
doi:10.1038/nature03345
Supplementary Data
Evidence (1) that the light-activated current in melanopsin expressing HEK293-TRPC3 cells is carried by TRPC3 channels; and (2) that the signaling cascade shares components with the M1 muscarinic receptor cascade. It discusses effects on the light response of altering or eliminating supplemental retinoids.
Supplementary Methods
Additional details on cell culture, expression of melanopsin, electrophysiological and pharmacological techniques, photic stimulation methods, spectral analysis, and calcium imaging.
Supplementary Figure 1
Light evokes calcium responses in HEK293-TRPC3 cells that express melanopsin but not EGFP.
Supplementary Figure 2
Evidence that (A) U73343, the inactive analog of the PLC blocker U73122, was without effect; (B) bath application of lanthanum abolishes the light response; and (C) inward currents evoked by the M1 muscarinic agonist carbachol occlude the light response in melanopsin-expressing HEK293-TRPC3 cells.
Supplementary Figure 3
Cumulative irradiance-response functions at the six wavelengths tested, pooled across all recorded cells.
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