Supplementary information

From the following article:

Structure and different conformational states of native AMPA receptor complexes

Terunaga Nakagawa, Yifan Cheng, Elizabeth Ramm, Morgan Sheng and Thomas Walz

Nature 433, 545-549(3 February 2005)

doi:10.1038/nature03328

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Supplementary Data

This file contains Supplementary Methods and legends for Supplementary Figures 1-5 and Table 1.

Supplementary Figure 1

Purification of AMPA-Rs.

Supplementary Figure 2

Plot of the angle distributions and comparison of reprojections from 3D models with the corresponding raw particle images.

Supplementary Figure 3

3D density map of AMPA-R in the type I conformation filtered to 42 Å (FSC = 0.5 criterion) or 31 Å (FSC = 0.142 criterion).

Supplementary Figure 4

ClustalW alignment of mGluR1 (extracellular domain), LIVBP, and GluR2-NTD.

Supplementary Figure 5

Class averages of particles obtained under different drug treatments.

Supplementary Table 1

Peptides and corresponding proteins identified by LC/MS/MS tandem mass spectrometry analysis of the bands indicated by asterisks in Fig. 3a.

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