Supplementary information

From the following article:

The simplicity of metazoan cell lineages

Ricardo B. R. Azevedo, Rolf Lohaus, Volker Braun, Markus Gumbel, Muralikrishna Umamaheshwar, Paul-Michael Agapow, Wouter Houthoofd, Ute Platzer, Gaëtan Borgonie, Hans-Peter Meinzer and Armand M. Leroi

Nature 433, 152-156(13 January 2005)

doi:10.1038/nature03178

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Supplementary Movie

The spatial positions of cells in the Pellioditis marina embryo are largely determined by the cell lineage. The movie shows a simulation of the embryonic development of P. marina based on 4D microscopy data (Supplementary Methods). Development runs for approximately 530 minutes from shortly after the third cell division (4-cell stage) until the onset of muscle contraction (571-cell stage). Initially, the embryo is viewed from the ventral side, with anterior to the left, and then rotates to a left lateral position during elongation. Cells are coloured in a gradient from red to blue corresponding to their position in the lineage diagram from left to right (Supplementary Fig. S3). Cells undergoing programmed cell death turn white and become increasingly transparent before vanishing.

Supplementary Methods

Details on cell lineage data, lineage complexity metric, and software. Additional references are included.

Supplementary Figures S1–6

Legends and references to accompany the below figures are also included in this file. Supplementary Fig. S1: our conclusions are robust to the way in which cells are classified. Supplementary Fig. S2: distributions of the effects of mutations on the complexity and lineage positions of cells for each of the metazoan lineages. Supplementary Fig. S3: the spatial positions of cells in the P. marina embryo are determined by the cell lineage. Supplementary Fig. S4: an extremely simple lineage capable of generating the terminal cells of the Caenorhabditis elegans embryo. Supplementary Fig. S5: the constraint on cell position is modeled as a selective constraint. Supplementary Fig. S6: the spatial constraint on the evolution of lineage complexity is not caused by an indirect reduction of population size.

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