FIGURE 2. Relationship between Fbxw7 and p53.
From the following article:
Fbxw7/Cdc4 is a p53-dependent, haploinsufficient tumour suppressor gene
Jian-Hua Mao, Jesus Perez-losada, Di Wu, Reyno DelRosario, Ryosuke Tsunematsu, Keiichi I. Nakayama, Ken Brown, Sheila Bryson and Allan Balmain
Nature 432, 775-779(9 December 2004)
doi:10.1038/nature03155
a, Fbxw7 mRNA expression in MEFs after irradiation (asterisk indicates P < 0.05). b, Fbxw7 RNA downregulation by siRNA. c, siRNA against Fbxw7 caused a delay in replicative senescence. Growth was prolonged by Fbxw7 siRNA, but the MEFs were not immortalized. Diamonds, wild-type MEFs; squares, wild-type MEFs with Fbxw7 siRNA control; triangles, wild-type MEFs with Fbxw7 siRNA. d, MEFs from p53 wild-type mice (right) and with Fbxw7 siRNA (left). e, Downregulation of Fbxw7 in p53+/- MEFs, but not in p53-/- MEFs, induced a growth advantage. Diamonds, p53+/- MEFs; open circles, p53+/- MEFs with Fbxw7 siRNA control; triangles, p53+/- MEFs with Fbxw7 siRNA; crosses, p53-/- MEFs; stars, p53-/- MEFs with Fbxw7 siRNA control; filled circles, p53-/- MEFs with Fbxw7 siRNA. f, Western blot showing Fbxw7 target protein levels in MEFs. Notch4 and c-Jun levels were upregulated when Fbxw7 was downregulated by siRNA in p53+/+ and p53+/- MEFs. No changes were found in Notch1, Presenilin 1 (Psen1) and cyclin E. g, Western blot showing increased Aurora-A levels when Fbxw7 is downregulated by siRNA in p53+/+ or p53+/- MEFs or keratinocytes. h, Aurora-A levels are upregulated in embryonic day 9.5 Fbxw7+/- and Fbxw7-/- MEFs. Controls were empty vector or constructs expressing other siRNA unrelated to Fbxw7. Error bars indicate standard deviation for three independent assays.
