Poly(ADP-ribose) is required for spindle assembly and structure

doi:doi:10.1038/nature03061

From the following article:

Poly(ADP-ribose) is required for spindle assembly and structure

Paul Chang, Myron K. Jacobson and Timothy J. Mitchison

Nature 432, 645-649(2 December 2004)

doi:10.1038/nature03061

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Supplementary Figure S1

PAR Localization to the kinetochore. Xenopus extract spindles were incubated with Alexa 488-labeled PAR antibody, and X-rhodamine-labeled CENPE antibody (CENP) and, visualized using spinning disk confocal microscopy every 30 s for 20 min. PAR co-localized with CENPE at every time point. In Merge, PAR is green and CENPE, red.

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Supplementary Movie M1

Real time spinning disk confocal microscopy of Xenopus egg extract spindles treated with 100 micro g/ml PARG. Images were obtained every 30s for 15 min.

Supplementary Movie M1 - Download Audio file (157KB)

Supplementary Movie M2

Real time spinning disk confocal microscopy of Xenopus egg extract spindles treated with 500 micro g/ml anti PAR antibody. Images were obtained every 30s for 15 min.

Supplementary Movie M2 - Download Audio file (187KB)

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