FIGURE 1. PAR is enriched in the spindle.
From the following article:
Poly(ADP-ribose) is required for spindle assembly and structure
Paul Chang, Myron K. Jacobson and Timothy J. Mitchison
Nature 432, 645-649(2 December 2004)
doi:10.1038/nature03061
a, PAR localization in purified Xenopus spindles. Xenopus egg extract spindles were isolated and stained for PAR, tubulin and DNA, without fixation (no fix), or after fixing with 4% paraformaldehyde (fix). PAR localizes throughout the spindle and is enriched at kinetochores and spindle poles (Supplementary Fig. S1). PAR appears filamentous when not fixed, and appears more punctate after fixation. In the merged panel, PAR is green, tubulin red and DNA is blue. b, Mitotic localization of PAR in somatic cells. HeLa, U2OS, LLPCK, XLK and BSC1 cells were fixed and stained for PAR, tubulin and DNA. All cell types exhibited similar staining patterns. HeLa cells during G2-M, prophase, prometaphase (P-M), metaphase, anaphase and cytokinesis stages of the cell cycle are shown. PAR localizes to the mitotic spindle throughout mitosis and appears filamentous during metaphase, similar to the staining pattern seen in isolated Xenopus egg extract spindles, which are arrested in metaphase. In the merged panel, PAR is green, tubulin red and DNA is blue. c, PARG localizes to the mitotic spindle. HeLa cells were stained with antibodies against PARG and tubulin. PARG co-localizes with tubulin, as shown in the merged panel (PARG is green, tubulin red and DNA is blue). d, PAR is enriched in the spindle. Immunoblot of equivalent amounts of CSF extract or isolated spindles (Spd) probed with anti-PAR antibody. Molecular mass is marked along the left (kDa).
