Supplementary information
From the following article:
A pancreatic islet-specific microRNA regulates insulin secretion
Matthew N. Poy, Lena Eliasson, Jan Krutzfeldt, Satoru Kuwajima, Xiaosong Ma, Patrick E. MacDonald, Sébastien Pfeffer, Thomas Tuschl, Nikolaus Rajewsky, Patrik Rorsman and Markus Stoffel
Nature 432, 226-230(11 November 2004)
doi:10.1038/nature03076
Supplementary Figure 1
Oligoribonucleotide 2'-O-me-375 anneals to endogenous miR-375 in MIN6 cells. Northern blot of MIN6 cells that were transfected with either 2'O-me-eGFP (control) or 2'-O-me-375.
Supplementary Figure 2
Increased [ATP]i levels in MIN6 cells infected with Ad-375 compared to Ad-eGFP. Total ATP measurements in MIN6 cells that were transfected with Ad-375 or Ad-eGFP.
Supplementary Figure 3
No apparent effects of miR-375 overexpression on submembrane actin network.
Structural analysis using fluorescence microscopy of the submembrane actin network in MIN6 cells and primary 
-cells that were infected with Ad-eGFP or Ad-375.
Supplementary Figure 4
Increased number of docked granules in Ad-375 infected -cells.
Electron micrographs of -cells infected with Ad-eGFP or Ad-375 and quantitative analysis of granule density.
Supplementary Table 1
Mature and precursor sequences of mouse (mmu-miR) and human (hsa-miR) novel miRNAs, identified in mouse pancreatic 
- and -cell lines TC1 and MIN6, respectively.
Supplementary Table 2
Foldback precursor sequences of novel mouse miRNAs, identified in mouse pancreatic -cell line MIN6.
Supplementary Table 3
Similar [Ca++]i in primary -cells and MIN6 cells infected with Ad-GFP and Ad-375.
Ca2+-measurements in primary -cells and MIN6 cells infected with Ad-GFP and Ad-375 in response to 25 mM glucose and to 30 mM K+.
Supplementary Table 4
Similar [Ca++]i in response to glucose and K+ in primary b-cells and MIN6 cells infected with control siRNAs, si-375 and si-Mtpn. Ca2+-measurements in MIN6 cells co-transfected with CMV-eGFP and si-ApoM (control), si-375 or si-Mtpn in response to glucose K+.
Supplementary Methods
Description of methods used to identify miR-375 targets.
DOWNLOAD BROWSER PLUGINS AND OTHER APPLICATIONS
Movie files
PDF douments
Text documents
PostScript documents
Flash movies
Audio files
- Apple iTunes (PC or Mac)
- QuickTime Player (PC or Mac)
- Realplayer (PC or Mac)
- Windows Media player (PC only)