FIGURE 2. In vivo silencing of murine apoB mRNA by siRNAs in wild-type mice.
From the following article:
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
Jürgen Soutschek, Akin Akinc, Birgit Bramlage, Klaus Charisse, Rainer Constien, Mary Donoghue, Sayda Elbashir, Anke Geick, Philipp Hadwiger, Jens Harborth, Matthias John, Venkitasamy Kesavan, Gary Lavine, Rajendra K. Pandey, Timothy Racie, Kallanthottathil G. Rajeev, Ingo Röhl, Ivanka Toudjarska, Gang Wang, Silvio Wuschko, David Bumcrot, Victor Koteliansky, Stefan Limmer, Muthiah Manoharan and Hans-Peter Vornlocher
Nature 432, 173-178(11 November 2004)
doi:10.1038/nature03121
Treatment groups comprised saline control (n = 10), chol-luc-siRNA control (n = 10), chol-mismatch-siRNA control (n = 10), unconjugated apoB-1-siRNA (n = 10), chol-apoB-1-siRNA (n = 10) and chol-apoB-2-siRNA (n = 7). bDNA measurements were performed with probe set II. Error bars represent the standard deviation (s.d.) of the mean. Statistical analysis was by analysis of variance (ANOVA) with Bonferroni post-hoc t-test, one-tailed. Asterisk, P < 0.0001 compared with saline control animals. a, Schematic representation of the apoB mRNA illustrating the binding regions of three bDNA probe sets in relation to the two siRNA cleavage sites. b, Effects of siRNA administration on mean apoB mRNA levels. c, apoB mRNA levels from individual mice treated with saline or siRNAs. Data are mean values from three liver samples from each individual animal. d, Effects of siRNA administration on the reduction of apoB mRNA measured by bDNA assays using three different probe sets.