FIGURE 3. Drosha and Pasha co-exist in a Microprocessor complex.
From the following article:
Processing of primary microRNAs by the Microprocessor complex
Ahmet M. Denli, Bastiaan B. J. Tops, Ronald H. A. Plasterk, René F. Ketting and Gregory J. Hannon
Nature 432, 231-235(11 November 2004)
doi:10.1038/nature03049
a, Combined nuclear and cytoplasmic extracts from S2 cells were loaded onto a Superose 6 column, and fractions were subjected to western blotting with Drosha or Pasha antibodies (lower panel). The Drosha antibody was used to recover Drosha complexes from each fraction and these were tested for their ability to process pri-bantam (upper panel). Positions of pre-bantam and the 5' and 3' flanks are indicated. b, Extracts prepared as in a were run on a Q sepharose FF column. Drosha and Pasha fractionation profiles were determined by western blotting (lower panels). Pri-miRNA processing activity on pri-bantam was examined using Drosha immunoprecipitates from each fraction. Direct analysis of processing activity in each fraction showed a similar processing activity profile, albeit with weaker activity than exhibited by the immunoprecipitates. c, Drosha peak fractions from a Q-sepharose column were pooled and loaded onto a phenyl HP column. Fractionation of Drosha and Pasha and processing activity were followed as in b.