FIGURE 1. Functional cloning and validation of TrkB as a suppressor of anoikis.

a, Parental or Ras^V12-expressing RIE cells seeded into ULC tissue culture plates and photographed at 50 magnification after 3 days (top). The bottom panel shows subsequent transfer of the cells to adhesive tissue culture plates, followed by fixation and crystal violet staining 2 days later. b, Outline of the screen. See Methods for details. c, Suppression of anoikis by clone ME-3, relative to a GFP-control, photographed at 50 (top) and 1 (bottom) magnification and at indicated time points. d, RIE cells expressing cDNAs as indicated, seeded on adhesive plates and photographed at 200 magnification. e, RIE cells expressing cDNAs as indicated, seeded into ULC plates and photographed in time at 50 (day 3) and 1 (day 14) magnification. f, Single cell suspensions of RIE cells expressing cDNAs as indicated, seeded into soft-agar and photographed at 50 magnification 1 week later.