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FIGURE 1. Measurement of kf and ku in the G48M and G48V Fyn SH3 domains.

From the following article:

Low-populated folding intermediates of Fyn SH3 characterized by relaxation dispersion NMR

Dmitry M. Korzhnev, Xavier Salvatella, Michele Vendruscolo, Ariel A. Di Nardo, Alan R. Davidson, Christopher M. Dobson and Lewis E. Kay

Nature 430, 586-590(29 July 2004)

doi:10.1038/nature02655

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a, Ribbon diagram of the wild-type Fyn SH3 domain (pdb accession code 1SHF; ref. 27), highlighting the site of mutation, Gly 48. b, c, Values of kf and ku for selected residues (labelled with residue number) from fits of dispersions assuming a two-state model at 15 and 35 °C for G48M (b) and 10 and 25 °C (G48V) (c). Errors in rates were measured with a jacknife procedure by which 25% of the points in each dispersion were randomly removed; subsequent fits of the data produce the ranges of rates indicated. Insets show representative dispersion curves (for Ala 12) recorded at 800 MHz (red) and 600 MHz (green) at 25 °C, with R2* the measured relaxation rate and nuCPMG the field strength employed.

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