1. Department of Biology and Biochemistry, University of Bath, BA2 7AY Bath, Somerset, UK
2. Collegium Budapest (Institute for Advanced Study) Szentháromság utca 2, Budapest H-1014, Hungary
3. MTA, Ecology and Theoretical Biology Research Group, Eötvös Loránd University, Pázmány Péter Sétány 1/C, Budapest H-1117, Hungary

Correspondence to: Laurence D. Hurst¹ Email: l.d.hurst@bath.ac.uk

Abstract

Under laboratory conditions 80% of yeast genes seem not to be essential for viability¹. This raises the question of what the mechanistic basis for dispensability is, and whether it is the result of selection for buffering or an incidental side product. Here we analyse these issues using an in silico flux model^{2, 3, 4, 5} of the yeast metabolic network. The model correctly predicts the knockout fitness effects in 88% of the genes studied⁴ and in vivo fluxes. Dispensable genes might be important, but under conditions not yet examined in the laboratory. Our model indicates that this is the dominant explanation for apparent dispensability, accounting for 37–68% of dispensable genes, whereas 15–28% of them are compensated by a duplicate, and only 4–17% are buffered by metabolic network flux reorganization. For over one-half of those not important under nutrient-rich conditions, we can predict conditions when they will be important. As expected, such condition-specific genes have a more restricted phylogenetic distribution. Gene duplicates catalysing the same reaction are not more common for indispensable reactions, suggesting that the reason for their retention is not to provide compensation. Instead their presence is better explained by selection for high enzymatic flux.

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