Supplementary information
From the following article:
An autonomous molecular computer for logical control of gene expression
Yaakov Benenson, Binyamin Gil, Uri Ben-Dor, Rivka Adar and Ehud Shapiro
Nature 429, 423-429(27 May 2004)
doi:10.1038/nature02551
Supplementary Methods
This file contains all the experimental protocols relevant to the main text, the detailed description of the automata design and the deoxyoligonucleotide sequences of its parts.
Supplementary Data
Three additional experiments are described: 1. Demonstration of the automaton ability to detect a point mutation; 2. Adjusting confidence in a positive diagnosis for various concentrations of the molecular indicator 3. The release of an approved ssDNA drug (Vitravene).
Supplementary Notes
A probabilistic framework for the diagnostic process is given.
Supplementary Figure 1
Architecture of the molecular finite automaton, featuring its input, software and hardware components.
Supplementary Figure 2
Molecular components of the computer.
Supplementary Figure 3
Calibration curve showing regulation of probability of Yes output state in a single-step computation by a pTRI-Xef generic mRNA marker.
Supplementary Figure 4
Selectivity of the diagnostic automata for their disease models.
Supplementary Figure 5
Release of the approved antisense drug.
Supplementary Figure Legends
DOWNLOAD BROWSER PLUGINS AND OTHER APPLICATIONS
Movie files
PDF douments
Text documents
PostScript documents
Flash movies
Audio files
- Apple iTunes (PC or Mac)
- QuickTime Player (PC or Mac)
- Realplayer (PC or Mac)
- Windows Media player (PC only)
