Note Best alignment is between forward est and forward genome, and splice  sites imply forward gene
Exon        70  98.6  3037  3108 PTB-056N14    1176  1247 hsa21-POTE_NM-174981  
?Intron    -40   0.0  3109  4512 PTB-056N14  
Exon        47 100.0  4513  4559 PTB-056N14    1248  1294 hsa21-POTE_NM-174981  
+Intron    -20   0.0  4560  5601 PTB-056N14  
Exon       163  98.8  5602  5768 PTB-056N14    1295  1461 hsa21-POTE_NM-174981  
+Intron    -20   0.0  5769 14202 PTB-056N14  
Exon       120  98.4 14203 14326 PTB-056N14    1462  1585 hsa21-POTE_NM-174981  
+Intron    -20   0.0 14327 16018 PTB-056N14  
Exon       235  98.8 16019 16259 PTB-056N14    1586  1826 hsa21-POTE_NM-174981  

Span       535  98.8  3037 16259 PTB-056N14    1176  1826 hsa21-POTE_NM-174981  

Segment     70  98.6  3037  3108 PTB-056N14    1176  1247 hsa21-POTE_NM-174981  
Segment     47 100.0  4513  4559 PTB-056N14    1248  1294 hsa21-POTE_NM-174981  
Segment    163  98.8  5602  5768 PTB-056N14    1295  1461 hsa21-POTE_NM-174981  
Segment    120  98.4 14203 14326 PTB-056N14    1462  1585 hsa21-POTE_NM-174981  
Segment    235  98.8 16019 16259 PTB-056N14    1586  1826 hsa21-POTE_NM-174981  


PTB-056N14 vs hsa21-POTE_NM-174981:

          PTB-056N14   3037 CAGAACAAGACTTAAAGCTGACATCAGAGGAAGAGTCACAAAGGCTTAAA   3086
                            ||||||||||||||||||||||||||||||||||||||||||||||||||
hsa21-POTE_NM-174981   1176 CAGAACAAGACTTAAAGCTGACATCAGAGGAAGAGTCACAAAGGCTTAAA   1225

          PTB-056N14   3087 GTCAGTAAAAATAGCCAGCCAGaggca......tctgtAGAAAATGTCTC   4524
                            |||||| |||||||||||||||????? 1404 ?????||||||||||||
hsa21-POTE_NM-174981   1226 GTCAGTGAAAATAGCCAGCCAG................AGAAAATGTCTC   1259

          PTB-056N14   4525 AAGAACCAGAAATAAATAAGGACTGTGATAGAGAGgtata......tcaa   4559
                            |||||||||||||||||||||||||||||||||||>>>>> 1042 >>>>
hsa21-POTE_NM-174981   1260 AAGAACCAGAAATAAATAAGGACTGTGATAGAGAG...............   1294

          PTB-056N14   4559 gGTTGAAGAAGAAATAAAGAAGCATGGAAGTAATCCTGTCGGATTACCAG   5650
                            >|||||||||||||||||||||||||||||||||||||| ||||||||||
hsa21-POTE_NM-174981   1294 .GTTGAAGAAGAAATAAAGAAGCATGGAAGTAATCCTGTGGGATTACCAG   1343

          PTB-056N14   5651 AAAACCTGACTAATGGTGCCAGTGCTGGCAATGGTGATGATGGAGTAATT   5700
                            |||||||||||||||||||||||||||||||||||||||||||| |||||
hsa21-POTE_NM-174981   1344 AAAACCTGACTAATGGTGCCAGTGCTGGCAATGGTGATGATGGATTAATT   1393

          PTB-056N14   5701 CCACAAAGGAGGAGCAGAAAACCTGAAAATCAGCAATTTCCTGACACTGA   5750
                            ||||||||||||||||||||||||||||||||||||||||||||||||||
hsa21-POTE_NM-174981   1394 CCACAAAGGAGGAGCAGAAAACCTGAAAATCAGCAATTTCCTGACACTGA   1443

          PTB-056N14   5751 GAATGAAGAGTATCACAGgtaag......tctagTGACGAACAAAATGAT  14218
                            ||||||||||||||||||>>>>> 8434 >>>>>||| ||||||||||||
hsa21-POTE_NM-174981   1444 GAATGAAGAGTATCACAG................TGATGAACAAAATGAT   1477

          PTB-056N14  14219 ACCCGGAAACAACTTTCTGAAGAACAGAACACTGGAATATCACAAGATGA  14268
                            ||||||||||||||||||||||||||||||||||||||||||||||||||
hsa21-POTE_NM-174981   1478 ACCCGGAAACAACTTTCTGAAGAACAGAACACTGGAATATCACAAGATGA   1527

          PTB-056N14  14269 GATTCTGACTAATAAACAAAAGCAGATAGAAGTGGCTGAAAAGAAAATGA  14318
                            |||||||||||||||||||||||||||||||||||||||| |||||||||
hsa21-POTE_NM-174981   1528 GATTCTGACTAATAAACAAAAGCAGATAGAAGTGGCTGAACAGAAAATGA   1577

          PTB-056N14  14319 ATTCTGAGgtatt......cttagCTTTCTCTTAGTCATAAGAAAGAAGA  16044
                            ||||||||>>>>> 1692 >>>>>||||||||||||||||||||||||||
hsa21-POTE_NM-174981   1578 ATTCTGAG................CTTTCTCTTAGTCATAAGAAAGAAGA   1611

          PTB-056N14  16045 AGATCTCTTGCGTGAAAACAGCATGTTGCGGGAAGAAATTGCCATGCTAA  16094
                            |||||||||||||||||||||| |||||| ||||||||||||||||||||
hsa21-POTE_NM-174981   1612 AGATCTCTTGCGTGAAAACAGCGTGTTGCAGGAAGAAATTGCCATGCTAA   1661

          PTB-056N14  16095 GACTGGAACTAGATGAAACAAAACATCAGAACCAGCTAAGGGAAAATAAA  16144
                            ||||||||||||||||||||||||||||||||||||||||||||||||||
hsa21-POTE_NM-174981   1662 GACTGGAACTAGATGAAACAAAACATCAGAACCAGCTAAGGGAAAATAAA   1711

          PTB-056N14  16145 ATTTTGGAGGAAATTGAAAGTGTGAAAGAAAAGACTGATAAACTTCTAAG  16194
                            ||||||||||||||||||||||||||||||||||||||||||||||||||
hsa21-POTE_NM-174981   1712 ATTTTGGAGGAAATTGAAAGTGTGAAAGAAAAGACTGATAAACTTCTAAG   1761

          PTB-056N14  16195 GGCTATGCAATTGAATGAAGAAGCATTAACGAAAACCAATATTGAAGTAC  16244
                            ||||||||||||||||||||||||||||||||||||||||||| ||||||
hsa21-POTE_NM-174981   1762 GGCTATGCAATTGAATGAAGAAGCATTAACGAAAACCAATATTTAAGTAC   1811

          PTB-056N14  16245 AGTGGACAGCTTAGG  16259
                            |||||||||||||||
hsa21-POTE_NM-174981   1812 AGTGGACAGCTTAGG   1826


Alignment Score: 535